Inosinic Acid Dehydrogenase of Sarcoma 180 Cells

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Inosinic acid dehydrogenase of sarcoma 180 cells.

Inosinic acid dehydrogenase was partially purified from sarcoma 180 cells. The concentrations of each reactant, inosine 5’-phosphate, nicotinamide adenine dinucleotide, and K+, altered both the apparent K, and Vmaxvalues for each of the other reactants. Enzymatic activity was inhibited by both products of the reaction, xanthosine S-phosphate and reduced nicotinamide adenine dinucleotide; the in...

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Inosinic Acid Dehydrogenase Activity

ated in normal subjects and in patients with the LeschNyhan syndrome. A significant difference in activity was found between erythrocytes derived from normal controls (1.21±0.47 pmoles/hr per mg protein) and from 15 patients with the Lesch-Nyhan syndrome (6.72 ±6.23 pmoles/hr per mg protein). However, no difference in activity was demonstrable in muscle or leukocytes derived from normal and Les...

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Cytoxicity and Apoptotic Mechanism of Ruthenium(II) Amino Acid Complexes in Sarcoma-180 Tumor Cells

Over the past several decades, much attention has been focused on ruthenium complexes in antitumor therapy. Ruthenium is a transition metal that possesses several advantages for rational antitumor drug design and biological applications. In the present study, five ruthenium complexes containing amino acids were studied in vitro to determine their biological activity against sarcoma-180 tumor ce...

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Studies with mouse sarcoma 180.

Negative results are reported for five compounds tested against mouse Sarcoma 180. Twelve mice, 18 gm. Rockland and Carworth Farms mice, were used for each test. The tumors were implanted subcutaneously by trocar in the right axilla. When the implants were seven days old the compounds were in jected intraperitoneallytwice a day for seven days. The compounds were tested at the maximum non-lethal...

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Growth of Sarcoma 180 in adrenalectomized mice.

Sarcoma 180 grew for only 10-14 days when implanted in bilaterally adrenalectomized male Swiss mice. The tumors completely regressed within 30 days. Untreated adrenalectomized mice never had tumors larger than 15 mm. in diameter. Adrenalectomy of mice after the tumor was established also resulted in regression of Sarcoma 180. Daily injections of Cortisol acetate or corticosterone at dosages fro...

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ژورنال

عنوان ژورنال: Journal of Biological Chemistry

سال: 1968

ISSN: 0021-9258

DOI: 10.1016/s0021-9258(18)93184-4